Coding
Part:BBa_K4202009:Design
Designed by: Hongying LIN Group: iGEM22_ZJU-China (2022-09-30)
Amorphous Calcium CarbonateBinding Protein(ACCBP)(without signal peptite)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 93
Illegal AgeI site found at 356 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 562
Design Notes
We are not sure whether the protein will be scecreted extracellularly with signal sequence.Therefore, we designed two parts, one with signal sequence, and another without the signal sequence.BBa_K4202008 is the one without signal sequence.
Source
The sequence of this part was originally retrieved from GenBankDQ473430.1. The codons of this sequence was optimized by Genscript.