Coding

Part:BBa_K4202009:Design

Designed by: Hongying LIN   Group: iGEM22_ZJU-China   (2022-09-30)


Amorphous Calcium CarbonateBinding Protein(ACCBP)(without signal peptite)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 93
    Illegal AgeI site found at 356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 562


Design Notes

We are not sure whether the protein will be scecreted extracellularly with signal sequence.Therefore, we designed two parts, one with signal sequence, and another without the signal sequence.BBa_K4202008 is the one without signal sequence.


Source

The sequence of this part was originally retrieved from GenBankDQ473430.1. The codons of this sequence was optimized by Genscript.

References